Application of pulsed-magnetic field enhances non-viral gene delivery in primary cells from different origins

Primary cell lines are more difficult to transfect when compared to immortalized/transformed cell lines, and hence new techniques are required to enhance the transfection efficiency in these cells. We isolated and established primary cultures of synoviocytes, chondrocytes, osteoblasts, melanocytes, macrophages, lung fibroblasts, and embryonic fibroblasts. These cells differed in several properties, and hence were a good representative sample of cells that would be targeted for expression and delivery of therapeutic genes in vivo. The efficiency of gene delivery in all these cells was enhanced using polyethylenimine-coated polyMAG magnetic nanoparticles, and the rates (17–84.2%) surpassed those previously achieved using other methods, especially in cells that are difficult to transfect. The application of permanent and pulsating magnetic fields significantly enhanced the transfection efficiencies in synoviocytes, chondrocytes, osteoblasts, melanocytes and lung fibroblasts, within 5 min of exposure to these magnetic fields. This is an added advantage for future in vivo applications, where rapid gene delivery is required before systemic clearance or filtration of the gene vectors occurs.     

Sch?dlinosbek?mpfung

Ohne Zusammenfassung     

Laminar compressible flow in a tube

A two-dimensional solution for the velocity and pressure distributions in steady, laminar, isothermal flow of an ideal gas in a long tube is obtained as a double perturbation expension in β, the radius to length ratio, and ε, the relative pressure drop. It is found that simple approximations estimate the exact flow rate-pressure drop relationship accurately.     

Nachweis von Arsen

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Zum Nachweis der Bors?ure

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Tabakuntersuchung und Nicotinbestimmung

Ohne Zusammenfassung     

Intramolecular Heck reactions of aryl chlorides with alkynes

We have developed a reaction that affords the selective preparation of hexahydro-2H-pyrido[2,1-a]isoquinoline dienes, allenes, or alkenes via an intramolecular Heck cyclization of an aryl chloride with an alkyne. Tricyclic isoquinoline core structures of this nature are difficult to access by alternative methods. The unsaturated product formed can be partially controlled by choice of ligand and reaction solvent.